Table 1. Estimates of the dose required for a minimally solvable molecular-replacement data set to 2 Å resolution from lysozyme, the maximum heating-limited dose rate assuming a 10 K rise, the minimum time to collect the data set and the maximum required detector frame rate as a function of crystal size.

The dose required for an MR data set was calculated using the model of Holton & Frankel (2010 ▶) and the program available at http://bl831.als.lbl.gov/xtalsize.html.

Crystal size (µm)	Dose for MR set (MGy)	Frames	Maximum dose rate (MGy s−1)	Minimum collection time (s)	Maximum frame rate (Hz)
10	11.8	90	6.5	1.8	50
20	1.8	90	2.3	0.78	115
50	0.13	90	0.58	0.23	400
100	0.018	90	0.20	0.09	1000