Table 1.
Analysis of mitotic phenotype in day 3.5 + 1 untreated or nocodazole-treated embryos from heterozygous crosses
|
|
+/− cross
|
+/+ cross
|
|||||
|---|---|---|---|---|---|---|---|
| Untreated
|
+ Nocodazole
|
+ Nocodazole
|
|||||
| Normal
|
Null
|
P value
|
Normal
|
Null
|
P value
|
Normal
|
|
| No. of embryos | 23 (74%) | 8 (26%) | 21 (78%) | 6 (22%) | 21 (100%) | ||
| No. of cellsa | 110 | 120 | 0.12 | 98 | 100 | 0.56 | 80 |
| Mitotic indexab | 2.1 | 1.6c | 0.55 | 19 | 3c | 6.1 × 10−6 | 17 |
| No. of micronucleia | 1.1 | 7.4d | 3.6 × 10−7 | 1.2 | 17d | 3.6 × 10−7 | 0.65 |
In addition to the normal litter mates from the heterozygous crosses, normal embryos from +/+ crosses were used as further controls to ascertain the effects of nocodazole.
a
Average value per embryo.
b
Expressed as a percentage of cells in mitosis over total cell number.
c
P = 0.071 for the mitotic indices of untreated versus nocodazole-treated null embryos.
d
P = 0.039 for the number of micronuclei in untreated versus nocodazole-treated null embryos.
P values were derived using the student's t test.