Figure 2.

There are four NPAT spots in S phase cells and two NPAT spots in non-S phase cells. (A) WI38 cells were incubated with 50 μM BrdU for 3.5 h, fixed, and stained with a mouse anti-BrdU antibody (red) and the rabbit anti-NPAT antibody R48 (green). DNA was stained with DAPI (blue). (B) WI38 cells were incubated with BrdU for 20 min and then fixed and stained as described in A. 100–150 cells were analyzed for the number of NPAT spots and BrdU staining status. The mean results from three independent experiments are depicted. The bars represent the standard deviations.