Table 1.
Cell cycle distribution of vector and NPAT transfected cells
|
|
U2OS
|
SAOS2
|
||||
|---|---|---|---|---|---|---|
| G1
|
S
|
G2/M
|
G1
|
S
|
G2/M
|
|
| Vector | 61.6 | 30.6 | 8.3 | 41.2 | 25.3 | 33.5 |
| NPAT | 61.2 | 29.8 | 9.0 | 40.3 | 26.5 | 33.2 |
U2OS or SAOS2 cells transfected with pCMV (vector) or pCMV–NPAT as indicated. The cells were harvested at 22 h (U2OS) or 36 h (SAOS2) after transfection, and the distributions of transfected cells in the cell cycle were analyzed by FACS as previously described (Zhao et al. 1998). When measured by BrdU incorporation as described in Materials and Methods, 38% of both vector- and NPAT-transfected U2OS cells are in S phase, while 30% of both vector- and NPAT-transfected SAOS2 cells are in S phase. The data represent the mean results from two independent experiments.