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. Author manuscript; available in PMC: 2011 Sep 8.
Published in final edited form as: Int J Antimicrob Agents. 2008 Sep 7;33(1):14–20. doi: 10.1016/j.ijantimicag.2008.05.021

Fig. 1.

Fig. 1

An overview of the procedure used to prepare the Porphyromonas gingivalis IgG-SMAP28 conjugate. Antiserum to whole cells of P. gingivalis was prepared in rabbits. Specific cell surface antibodies were then isolated with an immunoaffinity column and specific IgG was isolated using a protein G column. SMAP28 was then synthesised and a maleimide linker was attached. SMAP28 with the maleimide linker was attached to the specific P. gingivalis IgG antibody and dialysed. The conjugate was then tested for specific antimicrobial activity. IgG, immunoglobulin G; SMAP28, sheep myeloid antimicrobial peptide 28.