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. Author manuscript; available in PMC: 2011 Sep 8.
Published in final edited form as: Int J Antimicrob Agents. 2008 Sep 7;33(1):14–20. doi: 10.1016/j.ijantimicag.2008.05.021

Fig. 2.

Fig. 2

Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) showing that the maleimide linkers were successfully attached to sheep myeloid antimicrobial peptide (SMAP) 28. Up to four distinct peaks were seen. Each peak corresponded to the molecular mass of SMAP28 (i.e. 3198.95 Da) and the number of maleimide linkers attached (i.e. 219.24 Da). Predicted masses were 3418.19 Da (SMAP28 with one maleimide linker), 3637.43 Da (SMAP28 with two maleimide linkers), 3856.67 Da (SMAP28 with three maleimide linkers) and 4075.91 Da (SMAP28 with four maleimide linkers). Distinct peaks were seen at 3419.54, 3638.75, 3857.80 and 4077.32 m/z. Proteins with masses of 2867.81 Da and 5734.61 Da were used as internal calibration standards.