Table 2. Summary of protein-expression level and solubility from the cell-free expression system using small-scale expression, the Protemist DT II and the Protemist XE robotic platforms.
Conditions included WEPRO1240H and 7240H cell-free extracts in the presence and absence of NVoy. 1240H, WEPRO1240H extract; 7240H, WEPRO7240H extract; (−), without NVoy; (+), with 1 mg ml−1 NVoy.
| Small-scale screening | Protemist DT II | Protemist XE | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1240H, (−) | 1240H, (+) | 1240H, (−) | 1240H, (+) | 7240H, (−) | 7240H, (+) | 1240H, (−) | 1240H, (+) | 7240H, (−) | 7240H, (+) | |
| No. of targets | 44 | — | 29 | 5 | 6 | 5 | 4 | 1 | 4 | 2 |
| Any expression (%) | 87 | — | 97 | 80 | 67 | 80 | 100 | 100 | 100 | 100 |
| Soluble expression (%) | 56 | — | 59 | 80 | 17 | 80 | 75 | 100 | 50 | 100 |