Figure 3.

Reconstitution by coexpression of telomerase RNA and TERT. Coexpression reactions contained TERT and the indicated telomerase RNA variant. (A) An equal volume of each reaction was assayed for telomerase activity with the primer (TG)₈TTG. Products corresponding to primer +1 and +6 nucleotides are indicated. (B) An equal volume of each reaction was analyzed for telomerase RNA by Northern blot hybridization. The asterisk indicates the ∼160-bp DNA fragment encoding the stem–loop IV deleted RNA, which does not encode a full-length telomerase RNA sequence (see Materials and Methods). (C) An equal volume of each lysate coexpression reaction was analyzed for TERT by SDS-PAGE and autoradiography.