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Reconstitution by addition of purified RNA to lysate-expressed TERT. All reactions contained the same TERT expression lysate and the indicated telomerase RNA variant. One hundred nanograms of purified RNA was added to 3 μl of TERT expression lysate, incubated for 30 min at 30°C, then assayed for telomerase activity with the primer (TG)8TTG. RNAs are in ∼40-fold molar excess of TERT. Products corresponding to primer +0, +1, and +6 nucleotides are indicated.
