Reaction mix components:	for a 25 µl total volume reaction per well.
SYBR Green (2X):	12.5 µl	(1X concentration)
Forward primer:	0.5 µl	(9 µM)
Reverse primer:	0.5 µl	(9 µM)
Ultra pure water:	7.5 µl
Template:	4.0 µl
TOTAL:	25.0 µl