Fig. 1.
LPS-induced cytokine expression in primary BMMs. RT real-time PCR showed relative mRNA expression levels for TNF (A), MIP-1α (B) and IL-1β (C). LPS induced cytokine expression in TLR4+/+ cells while demonstrating no cytokine induction in TLR4−/−. NFAT-specific inhibitor, VIVIT, inhibits gene expression of TNF (A), MIP-1α (B) and IL-1β (C) in BMMs stimulated with LPS. The relative mRNA expression levels were measured by RT real-time PCR with normalization to GAPDH levels. Symbol (*) denotes significant difference; p<0.05.