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. 1999 May 1;13(9):1140–1155. doi: 10.1101/gad.13.9.1140

Figure 7.

Figure 7

Figure 7

(A) Substitution of CDE I+II in a CEN3–HIS3 reporter construct interferes with AD–Cbf3a-promoted activation of HIS3 transcription. The reporter constructs of strain YJL162 (cen2::TRP1, LYS2::CEN3–HIS3) containing wild-type CEN3 DNA and strain YJL138 (LYS2::CDE III–HIS3) are presented. Growth of strains YJL162 (cen2::TRP1, LYS2::CEN3–HIS3) and YJL138 (LYS2::CDE III–HIS3) expressing AD–Cbf3a (pJO196) on SD/His− medium containing 5 mm 3-AT is shown. (B) S. cerevisiae kinetochore model. Solid lines, broken lines, and arrows represent protein–protein interactions revealed by experimental methods as indicated. For further explanation of the model, see text.