Table 2.

PCR primers used in the Chip assay

Fragment amplifieda	Fragment size (bp)	Primers used	Primer origin
CEN3WT–HIS3	275	AACTTCCACCAGTAAACGTTTC	flanking CEN3
		CTTCGTTTATCTTGCCTGCTC	HIS3
CEN3AGC–HIS3	275	as above
CDEIII–HIS3	275	as above
CEN3WT	243	AACTTCCACCAGTAAACGTTTC	flanking CEN3
		GATCAGCGCCAAACAATATGG	flanking CEN3
III–L	213	ACTTTGGCTTTCCGCTCGTG	Chromosome III
		GAAAGTCTTCTAGAGTTACAGG	Chromosome III
III–R1	321	GACCAGCATGTAGGAAGGTG	Chromosome III
		ACATTGATAAATTGCTCTCACCA	Chromosome III
III–R2	278	ATCGCATTTCTTTTCGTCCACA	Chromosome III
		TTGGACGGGGATCGTTCGTA	Chromosome III

^aSee also Figs. 5 and 6.