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. 2011 Aug 11;12:46. doi: 10.1186/1471-2172-12-46

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Copyright ©2011 Vintiñi and Medina; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoglobulin (Ig)A (a) and IgG (b) anti-pneumococcal protective protein A (PppA) antibodies response in nasal and bronchoalveolar lavages (NL and BAL) and serum of young mice nasally immunized with PppA and PppA associated with live (LcV) and heat-killed (LcM) Lactobacillus casei CRL 431. Results are expressed as the mean of the optical density (OD) ± standard deviation for each specific Ig (n = 6 mice/group at each time point). Antibodies were determined using the enzyme-linked immunosorbent assay method and samples were considered negative for the presence of specific antibodies when OD 493 < 0.1. Results are representative of two independent experiments. Significant differences with the PppA group were: P < 0.01.