Table 3.
Primers and probes used for qPCR analyses
| Genes | Assay | Primer | Sequence (5'-3') | PCR efficiency | GenBank accession # |
|---|---|---|---|---|---|
| EF1aB | TaqMan | Forward Reverse Probe |
TGCCCCTCCAGGATGTCTAC CACGGCCCACAGGTACTG AAATCGGCGGTATTGG |
2.0 | BG933897 |
| IFN-c | TaqMan | Forward Reverse Probe |
TGGGCAGTGTGGATACAAGTG CTGCAATGTTCCCAAAGTACGTATT CTGTCCTGATGAGATAAT |
2.0 |
EU735545 EU735547-50 |
| IFN-a1 | TaqMan | Forward Reverse Probe |
CCTTTCCCTGCTGGACCA TGTCTGTAAAGGGATGTTGGGAAAA CTTTGTGATATCTCCTCCCATC |
1.94 |
AY2169594 AY2169595 |
| IFN-γ | TaqMan | Forward Reverse Probe |
AAGGGCTGTGATGTGTTTCTG TGTACTGAGCGGCATTACTCC TTGATGGGCTGGATGACTTTAGGA |
2.0 | AY795563 |
| Mx1/2 e/e | TaqMan | Forward Reverse Probe |
GATGCTGCACCTCAAGTCCTATTA CGGATCACCATGGGAATCTGA CAGGATATCCAGTCAACGTT |
2.0 |
U66475 U66476 |
| MyD88 | TaqMan | Forward Reverse Probe |
GACAAAGTTTGCCCTCAGTCTCT CCGTCAGGAACCTCAGGATACT CTGGTGCCCGGAGCAA |
1.84 | EF672332 |
| TLR8 | TaqMan | Forward Reverse Probe |
ACCAAAACCACTAATGACATCATCTTCA TGGTGATGCCATCAGGTATGTTT CTCAGTCGACGCTCCTC |
1.7 | FJ467615 |
| TLR9 | TaqMan | Forward Reverse Probe |
TCTATGGCTGGGATGTCTGGTA CAGTTGTGAGTAGCCCTTGTGT CAGCACCTGGAAGCAG |
1.81 | EF672331 |
| TLR22 | TaqMan | Forward Reverse Probe |
ATTTATCCCGGAATCCATGTATCACG CCACAGTAGGCGATGTCTAACA CCTCAAGATAAGGAAGAACAT |
1.98 | AM233509 |
| VP2 (IPNV) | TaqMan | Forward Reverse Probe |
GCCAAGATGACCCAGTCCAT TGACAGCTTGACCCTGGTGAT CCGACCGAGAACAT |
2.0 | AJ877117 |
| Beta 2 microglobulin | SybrGreen | Forward Reverse |
TCGTTGTACTTGTGCTCATTTACAGC CAGGGTATTCTTATCTCCAAAGTTGC |
1,6 | BX076608 |
| Galectin-3 binding protein | SybrGreen | Forward Reverse |
CCAGACCAACAGTGTTCACTTCAGC ACGTGAAAGACATACCTGCCCTCAC |
1,84 | BX079375 |
| Galectin-9 | SybrGreen | Forward Reverse |
GTCCTGTCTATTGCCTTCTCCAACC GGTTTCGTTGACCACTGTGTGGA |
1,76 | CU071943 |
| MHC-IHC | SybrGreen | Forward Reverse |
CTGCATTGAGTGGCTGAAGA GGTGATCTTGTCCGTCTTTC |
1,72 | CU070775 |
| Pre-B cell ef | SybrGreen | Forward Reverse |
GACTTCAATTTCCTGCTGGCTA CTGCTTGTAATGTGTGACCT |
1.77 | CA376673 |
| RIG-I | SybrGreen | Forward Reverse |
GACGGTCAGCAGGGTGTACT CCCGTGTCCTAACGAACAGT |
1.97 | DY714827 |
| MDA5 | SybrGreen | Forward Reverse |
CAGAGGTGGGGTTCAATGAT AGCTCGCTCCACTTGTTGAT |
1.92 | FN396357 |
| PKR | SybrGreen | Forward Reverse |
TTCCTGCATGGACTTGACTG GTGAGGAACCGGTGTTCTGT |
1.87 | BT046111 |
| SRK2 | SybrGreen | Forward Reverse |
TAGACATGGCACCATGGACCCTC GGGTTCTTCAGTGCAGACAGCCA |
1.81 | CA349577 |