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. 2011 Sep 8;6(9):e23482. doi: 10.1371/journal.pone.0023482

Figure 1. T. cruzi PCM inhibits agonist-induced ctgf/ccn2 expression.

Figure 1

HFF were incubated with medium or T. cruzi PCM, alone or in combination with 5 ng/ml TGF-ß, 2 hours prior to mRNA harvest for quantitative real-time PCR analysis (A) or 24 hours prior to analysis of CTGF/CCN2 protein levels by western blot (B). ctgf/ccn2 mRNA levels were analyzed by quantitative real-time PCR following stimulation of HFF with serum (2% v/v) (C) or 10 µM LPA (D) for 2 hours in the presence or absence of T. cruzi PCM. Data is represented as the mean ± S.E. from 5 independent experiments carried out in duplicate. Statistical significance was assessed using the Student's t-test, (** p<0.01).