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Cosupertransfection of STAT3F with wild-type STAT expression vectors. Proportions of undifferentiated stem cell colonies generated after cosupertransfection of MG1.19 ES cells with 10 μg of pBPCAGGS–STAT3F plus 10 μg of pHPCAG vector containing stuffer (control), STAT3, STAT1, or STAT4 inserts. After 8 days of selection with 80 μg/ml of hygromycin B plus 20 μg/ml of blasticidin S, colonies were fixed and stained with Leishman’s reagent.
