Figure 12. Phenolphthiocerol dimycocerosate does not influence TNF-α and IL-1β production from macrophages.

TLC analysis of lipids from Indo-Oceanic strain 367, its isogenic pks15/1::km mutant (PMM162) and pks15/1 complemented strain (PMM162:pPET1). Lipids were radiolabelled with ¹⁴C- propionate. The TLC was run in petroleum ether/diethylether (90:10, v/v) (A). Bone marrow derived macrophages were infected at an M.O.I of 5 for 72 hours with a representative Indo-Oceanic strain (no. 367) , mutant (PMM162) strain incapable of making lipid Y (pks15/1 knock-out mutant) and the complemented strain . Cytokine concentrations in the cell supernatants were determined using ELISA. TNF-α (B) and IL-1β concentrations (C) are shown across the 3 time-points studied. Data represents mean ± standard deviation (SD) of three independent experiments.