Skip to main content
. 2011 Sep 8;6(9):e23877. doi: 10.1371/journal.pone.0023877

Figure 5. Inhibition of in vitro capsid assembly by cocktails of interfacial peptides.

Figure 5

Reaction kinetics of CA polymerization using the conditions described in the legend for Fig. 3. (A) Inhibition by CAI under the macromolecular crowding conditions used for testing the inhibitory activity of the designed peptides described in this work. The traces are colored as follows: no peptide added (black); an inactive peptide at a peptide∶CA molar ratio of 10 (grey); CAI added at peptide∶CA ratios of 0.1 (light blue), 0.2 (blue), 0.3 (green), 0.4 (orange) or 0.5 (pink). (B) Inhibition by a cocktail of CAI and H8. No peptide added (black); an inactive peptide at a peptide∶CA ratio of 10 (grey); CAI added alone at a peptide∶molar ratio of 0.2 (blue); H8 added alone at a peptide∶CA molar ratio of 5 (orange); a cocktail of CAI and H8 added at the same molar ratios used for each peptide alone (pink). (C) Inhibition by a cocktail of CAC1M and H8. No peptide added (black); an inactive peptide at a peptide∶CA ratio of 10 (grey); H8 added alone at a peptide∶molar ratio of 5 (orange); CAC1M added alone at a peptide∶CA molar ratio of 2 (green); a cocktail of H8 and CAC1M added at the same molar ratios used for each peptide alone (pink). (D) Inhibition by a cocktail of CAC1M and CAI. No peptide added (black); an inactive peptide at a peptide∶CA ratio of 10 (grey); CAI added alone at a peptide∶molar ratio of 0.2 (blue); CAC1M added alone at a peptide∶CA molar ratio of 2 (green); a cocktail of CAI and CAC1M added at the same molar ratios used for each peptide alone (pink). (E) Inhibition by a cocktail of three peptides: CAC1M, CAI and H8. No peptide added (black); an inactive peptide at a peptide∶CA molar ratio of 10 (grey); CAI added alone at a peptide∶molar ratio of 0.2 (blue); CAC1M added alone at a peptide∶CA molar ratio of 2 (green); H8 added alone at a peptide∶molar ratio of 5 (orange); a cocktail of CAI, CAC1M and H8 added at the same molar ratios used for each peptide alone (pink).