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. 2011 Sep 8;6(9):e23877. doi: 10.1371/journal.pone.0023877

Figure 7. Ex vivo inhibition of HIV-1 infection by assembly inhibitor peptides.

Figure 7

Peptides H4 (as a negative control), CAC1, CAC1M, H8, CAI and the indicated peptide cocktails were incubated with Chariot and transported into cells, and the cells were infected with HIV-1 strain HXB2 (0.0015 TCID50/cell) as described in Methods. The concentrations of peptides used in the experiments shown were: H4, 0.8 mM (labelled H4l) and 1.7 mM (labelled H4h); CAC1, CAC1M, CAI (alone or as part of a cocktail), 170 µM; H8 (alone or as part of a cocktail), 2.6 mM. Supernatants were collected at 72 h after infection, and the amount of CA produced (p24 antigen) was determined as described in Materials and Methods. The values obtained were normalized to the amount of p24 obtained in the absence of any peptide added. 100% virus production corresponds to 37,000 pg/ml of p24 antigen. The average and standard error from equivalent data obtained in several experiments are indicated.