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. 2011 Sep 8;6(9):e24376. doi: 10.1371/journal.pone.0024376

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) RPE membrane proteins from 3-month-old mice were separated by SDS-PAGE, blotted and reacted with Gα_common antibodies. Protein bands were visualized using the ECL detection reagent. The RPE proteins are from: Gαi1-/- (lane 1), Gαi2-/- (lane 2), Gαi3-/- (lane 3), DKO (lane 4) and 129 Sv (lane 5) mice. The membrane was then stripped and reacted with α-tubulin antibodies to confirm that the amount of protein loaded on each lane was the same. B) Analysis of the immunoblot by densitometry using the Quantity One 1-D Software. Numbers represent the optical density units per mm² (OD U/mm²) of each Gαi protein band.