Table 1. Summary of ethanol and NAP effects on L1 mRNA and protein expression in three cerebellar culture systems.
Sample type | L1 mRNA (% RQ±SEM) | n | p | L1 protein (% RQ±SEM) | n | p |
Cerebellar slices | ||||||
4 hr | ||||||
Control | 100±13 | 7 | - | 100 | 6 | - |
EtOH | 102±9 | 7 | 0.92 | 82±16 | 6 | 0.30 |
NAP | 91±4 | 4 | 0.59 | 121±59 | 6 | 0.73 |
NAP+EtOH | 114±9 | 4 | 0.46 | 88±21 | 6 | 0.60 |
24 hr | ||||||
Control | 100±6 | 4 | - | 100 | 4 | - |
EtOH | 104±10 | 4 | 0.83 | 76±14 | 4 | 0.19 |
NAP | 101±7 | 4 | 0.91 | 98±26 | 4 | 0.94 |
NAP+EtOH | 99±4 | 4 | 0.85 | 112±33 | 4 | 0.75 |
10 day | ||||||
Control | 100±10 | 4 | - | 100 | 3 | - |
EtOH | 108±8 | 4 | 0.45 | 87±40 | 3 | 0.80 |
NAP | 97±3 | 4 | 0.94 | 100±28 | 3 | 0.99 |
NAP+EtOH | 65±7 | 4 | 0.03 | 113±42 | 3 | 0.78 |
CGNs | ||||||
4 hr | ||||||
Control | 100±12 | 5 | - | 100 | 4 | - |
EtOH | 115±13 | 5 | 0.39 | 109±19 | 4 | 0.66 |
NAP | 118±6 | 5 | 0.24 | 92±10 | 4 | 0.50 |
NAP+EtOH | 131±7 | 5 | 0.07 | 99±32 | 4 | 0.98 |
24 hr | ||||||
Control | 105±6 | 4 | - | 100 | 4 | - |
EtOH | 96±4 | 4 | 0.25 | 109±25 | 4 | 0.76 |
NAP | 103±4 | 4 | 0.77 | 81±10 | 4 | 0.16 |
NAP+EtOH | 98±7 | 4 | 0.48 | 83±11 | 4 | 0.22 |
Astrocytes | ||||||
4 hr | ||||||
Control | 100±10 | 3 | - | |||
EtOH | 85±13 | 3 | 0.47 | |||
NAP | 77±11 | 3 | 0.20 | |||
NAP+EtOH | 97±15 | 3 | 0.90 | |||
24 hr | ||||||
Control | 100±15 | 8 | - | 100 | 4 | - |
EtOH | 114±26 | 6 | 0.63 | 111±10 | 4 | 0.37 |
NAP | 84±20 | 5 | 0.52 | 132±26 | 4 | 0.31 |
NAP+EtOH | 150±57 | 3 | 0.23 | 109±22 | 4 | 0.72 |