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. 2011 Sep 8;6(9):e24392. doi: 10.1371/journal.pone.0024392

Figure 3. cAMP PDE activity in HEK293 cytosol and membrane fractions.

Figure 3

Lysate from either the cytosolic fraction (A) or the membrane fraction (B) was tested in the PDE activity with 1 µM cAMP. Selective PDE inhibitors were added alone or in combination as indicated. The results represent the mean ± SEM of three experiments. Compounds and concentrations used: PDE1i, 250 nM (see M&M section); PDE2i, 20 nM BAY 60-7550; PDE3i, 400 nM cilostamide; PDE4i, 50 nM roflumilast; PDE10i, 300 nM MP-10. The ratio of total cAMP PDE activity in the cytosolic fraction and membrane fraction compared to whole cells was 40.4±5.3% and 59.6±5.3%, respectively.