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. 1999 Aug 15;13(16):2108–2117. doi: 10.1101/gad.13.16.2108

Figure 4.

Figure 4

Nuclear localization of SPL–GUS fusion protein. Onion epidermis was bombarded with pBI221 DNA in A and pBI221–SPL–GUS construct in B. The pPZP111 construct containing 35S promoter and SPL–GUS fusion was introduced into Arabidopsis through Agrobacterium-mediated vacuum infiltration in C. Samples were stained for GUS activity. (A) Control GUS protein is distributed throughout the cell. (B) SPL–GUS fusion protein is localized to the nucleus (N). No GUS activity is detected in the cytoplasm. (C) SPL–GUS fusion protein is localized in the nucleus (N) in trichomes of a transgenic Arabidopsis plant. Bar, 10 μm.