Table 1. Bacterial strains and plasmids used in this study.
| Name | Relevant characteristics | Source/Ref |
| Francisella strains | ||
| Schu S4 | F. tularensis tularensis, wild-type | CDC |
| BJM1031 | Schu S4 ΔfipB | Qin [14] |
| BJM1068 | Schu S4 ΔfipA | This study |
| BJM1069 | Schu S4 DfipA + B | This study |
| BJM1076 | fipA+B+ in cis complement of ΔfipAB | This study |
| BJM1077 | fipA+B C164A in cis complement of ΔfipAB | This study |
| BJM1078 | fipA+B C167A in cis complement of ΔfipAB | This study |
| Plasmids | ||
| pMP815 | Chromosomal integration system vector | LoVullo [46] |
| pGIR463 | sacB suicide vector | Sullivan [44] |
| pAQ136 | 5′flanking region of fipA in GIR463 (BM248/BM249) | This study |
| pAQ137 | 5′-and 3′flanking regions of fipA in GIR463 (BM250/BM251) | This study |
| pAQ138 | 5′-and 3′ flanking regions of fipAB in GIR463 (BM256/BM085) | This study |
| pAQ162 | fipA+fipB+ in pMP815 | This study |
| pAQ163 | fipA+fipB C164A in pMP815 | This study |
| pAQ164 | fipA+fipB C167A in pMP815 | This study |