Skip to main content
. 2011 Sep 8;6(9):e24611. doi: 10.1371/journal.pone.0024611

Table 3. Primers used in this study.

Primer Sequence 5′-3′ Descript. Restr. Enz.
BM063 TCCATATGCAAGAAATGGCTGCTC F fipB NdeI
BM064 GCGGCCGCTATAAGAAGGATAGGC F fipA NotI
BM066 GGATCCTATCATCATCTTGGCTGAGC R fipB BamHI
BM150 CTTTGATTATCAAGCTATGTACTGTTCTAAGCTTGC F fipB C164A
BM151 GCAAGCTTAGAACAGTACATAGCTTGATAATCAAAG R fipB C164A
BM152 CAATGTATGTACGCTTCTAAGCTTGCTTGCTCC F fipB C167A
BM153 GGAGCAAGCAAGCTTAGAAGCTTACATACATTG R fipB C167A
BM245 AGAAAATATGCGGCCGCGAAATAATAGGAG F fipA NotI
BM208 TCCTCGAGCTTATTTCTTTTGAGCAGCC R fipA XhoI
BM248 GAGCCCTAGGTAGAACAATGGCAACAGG F fipA 5′deletion AvrII
BM249 AGGCGGCCGCATTATTTAGTTTCTCCTA R fipA 5′deletion NotI
BM250 ATAGATCAGCGGCCGCATGCAATGATTGAATTCC F fipA 3′deletion NotI
BM251 ATTAGAGCTCAACACTATCATCATCTTGGCTGAGC R fipA 3′deletion XhoI
BM256 CTAAGTCTGCGGCCGCAACAACTAGTACTAGC F fipAB 5′deletion NotI
BM085 CTCGAGATTACAGCATTACCAGCTGC F fipAB 3′deletion XhoI
BM297 GAGGAATAATAAATGAAATTAACTAAAACTCT F fipA
BM298 TTATTTCTTTTGAGCAGCCAT R fipA