Table 1. Statistics of screening antibodies.
| AdiC | CLC-ec2 | |||||||
| Immunization | group1 | group2 | group3 | group4 | group5 | group1 | group4 | |
| 1° ELISA(Initial positives) | 25 | 33 | 29 | 33 | 25 | 60 | 10 | |
| 2° ELISA1(Native≫unfolded) | 8/18 | 6/20 | 3/25 | 3/33 | 2/15 | 29/60 | 2/10 | |
| Subtypes | IgG1 | 0 | 6 | |||||
| IgG2a | 10 | 7 | ||||||
| IgG2b | 7 | 12 | ||||||
| IgG3 | 0 | 0 | ||||||
| IgA | 2 | 1 | ||||||
| IgM | 3 | 0 | ||||||
| Not determined | 0 | 5 | ||||||
| Complex formation2 | 22/22 | 12/31 | ||||||
Legend: The Table reports results of hybridoma production for 7 separate immunizations – 5 with AdiC and 2 with CLC-ec2.
1
Displayed are the numbers of clones showing >2-fold brighter ELISA signals in native than unfolded conditions over the number tested.
2
Number of antibodies showing a chromatographically satisfactory complex over total tested.