Figure 4.

Transfected cdr2 redistributes c-Myc to the cytoplasm of N2A cells where it colocalizes with cdr2 protein. N2A cells were transfected transiently with pcDNA3-cdr2 plasmid, fixed and stained with anti-cdr2 patient CSF (A, green, Cy2) and anti-Myc polyclonal C-19 antibody (B, red, Cy5), and imaged by confocal microscopy (Zeiss). Fusion of images in A and B shows overlap in the cytoplasmic expression of both proteins (C, yellow). Nontransfected cells (arrowheads) show little or no cdr2 staining and nuclear c-Myc expression; transfected cells (arrow) show strong cytoplasmic cdr2 staining and a redistribution of c-Myc reactivity to the cytoplasm, where it colocalizes with cdr2. The same result was seen when a different anti-c-Myc antibody (N262) was used (data not shown).