Table 4.

¹H NMR Chemical shift (δ, ppm), CIS (ppm) and temperature coefficients (dδ/dT, ppb/°C) for hydroxy protons of puerarin at −8°C in 85% H₂O / 15% acetone-d₆

	OH-2″	OH-3″ a	OH-4″ a	OH-6″
δ	6.559	6.621	6.621	6.338
CIS	0.008	0.012	0.012	0.021
dδ/dT	− 12.4	− 13.6	− 13.6	b
dδ/dT (β-CD)	− 12.1	− 13.7	− 13.7	b

^a the OH-3″ and OH-4″ signals were overlapping over the all range of temperature

b could not be determined