Skip to main content
. Author manuscript; available in PMC: 2012 Mar 8.
Published in final edited form as: Nature. 2011 Aug 21;477(7363):211–215. doi: 10.1038/nature10353

Fig. 3.

Fig. 3

Co-localization of ubiquilin2 and ALS- and dementia-linked TDP43. Neuro2a cells were transfected with various combinations of wild-type ubiquilin2, wild-type TDP43, mutant ubiquilin2 (P497H), and C-terminal fragment of TDP43 that is linked to ALS and FTLD. Ubiquilin2 is GFP-tagged and TDP43 is mCherry-tagged. Wild-type and mutant ubiquilin2 are largely cytoplasmic. WtTDP43 are almost exclusively distributed in the nuclei. The C-TDP43, an ALS- and dementia-linked TDP43 fragment (aa218-414) is almost exclusively cytoplasmic. TDP43 inclusions are co-localized with wild-type (g–i) and mutant (P497H) ubiquilin2 (j–l) (arrows). Some ubiquilin2-positive inclusions are TDP43-negative (arrowhead).