Requirements of EGFR and vein during endoderm induction. Side views of 12- to 14-hr-old embryos stained with Lab (A–E) or Ubx antibody (F, G) or with lacZ antibody to visualize Ubx B expression (H,I). Wild type (A,F,H); flb1F26 homozygote (B); 48Y.GAL4/UAS.DN-DER (C); 24B.GAL4/UAS.DN-DER (G,I); veinγ4 homozygote (D); veinγ3/veinP1749 (E). Note the loss of Lab staining and the reduced Ubx staining (▵ in B–E, G) and the loss of Ubx B-mediated expression in ps6/ps7 and in ps3 (▵ in I), owing to lack of function of EGFR or vein. Anterior to the left, dorsal up (orientation the same in all figures).