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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2011 Aug 10;187(6):2915–2922. doi: 10.4049/jimmunol.1000180

FIGURE 2.

FIGURE 2

IL17A-IRES-eGFP mice accurately report IL-17 production capacity of CD4 T cells. A Comparison of IL-17A detection by eGFP signal or by intracellular cytokine staining (ICS) for IL-17A using mAb. Splenocytes from day 1 LCMV-infected mice were restimulated in vitro for 3 hrs with PMA/ionomycin + Brefeldin A. Cells were fixed with BDFix, then stained for intracellular IL-17A and IFNγ by mAb in BDPerm buffer. B In vitro restimulation by PMA/ionomycin overestimates the in vivo production of IL-17A cytokine by CD4 T cells. eGFP levels were measured by flow cytometry after in vitro restimulation with PMA/ionomycin + Brefeldin A, or without restimulation.