IL-17A is not produced by T cells during an anti-LCMV response. A The in situ production of IL-17A by splenic T cells was determined at various time points following acute low dose LCMV infection (104 pfu LCMV Armstrong; panel A), acute high dose LCMV infection (2×106 pfu LCMV Armstrong; panel B, left side), or protracted infection (2×106 pfu LCMV Clone 13; panel B, right side) of IL-17A eGFP C57BL/6 mice. Cells were freshly stained for the indicated surface markers and freshly acquired without prior in vitro restimulation, inhibition of protein secretion, cell fixation or permeabilization. Graphs shown are gated on CD4 T cells (top rows) or CD8 T cells (lower three rows). n=4 per time point per infection condition. gp33-41/Db and np396-404/Db are pentamers to detect gp33-41-specific and np396-404-specific CD8 T cells, respectively. Numbers in graphs denote the percentages of cells in the respective gates.