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. 1998 Jul 1;12(13):1986–1997. doi: 10.1101/gad.12.13.1986

Figure 3.

Figure 3

Figure 3

 Cell cycle-dependent chromosomal targeting of cohesins in vivo and in vitro. (A) Cell cycle-dependent chromosomal targeting of cohesins and condensins. Sperm chromatin was incubated with an interphase low-speed supernatant for 90 min and then a non-degradable form of sea urchin cyclin B (cyc BΔ90) was added to drive the cell cycle into mitosis. Aliquots were taken at the indicated time points, and chromatin-bound protein fractions were isolated and analyzed by immunoblotting with antibodies against cohesin subunits (XSMC1, XSMC3, and XRAD21), condensin subunits (XCAP-C, XCAP-D2, XCAP-E, and XCAP-G) or topoisomerase IIα. The level of H1 kinase activity in the extract was also assayed. (B) Immunolocalization of XSMC3 in tissue culture cells. Xenopus XL177 tissue culture cells fixed with paraformaldehyde were stained with DAPI (left) and anti-XSMC3 (right). Bar, 10 μm.