Skip to main content
. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2011 Aug 10;187(6):3087–3095. doi: 10.4049/jimmunol.1003879

FIGURE 5. Resting NK cells do not kill tumor cells or DCs.

FIGURE 5

A, Splenocytes from RAG-I−/− mice were incubated with or without IL-4 (1,000 U) for 4 days, and then cocultured with P815/tOVA cells or YAC-1 cells at ratios from 1:1 to 25:1 in 5-h 51Cr release assays. Cumulative data from three independent experiments are shown as mean ± SEM. B, primary non-activated NK cells do not induce cell death of DCs. DCs (BM-DCs, 5×104) alone or NK (105) plus DCs were cultured in 96-well plates for either 24 hr or 48 hr before annexin V and PI staining. The data shown are gated on DCs (APC-anti-CD11c+). As positive control, 5% EtOH was added 24h before cell staining. A representative result of two independent experiments is shown as mean ± SEM of triplicates.