FIGURE 8. Ariginase-1 is involved in DR5-mediated inhibition of DC cross-priming.

(A) CD11c⁺ BM-DCs (5 × 10⁴) were cultured for 4 hr and 24 hr in control mAbs or anti-DR5 mAbs-coated 96-well plates or in control wells (PBS). The expression of indicated genes were quantified by real-time RT-PCR (PBS at 4 hr = 1). Cumulative data from two independent experiments are shown as mean ± SEM. (B) CFSE-labeled OVA-specific OT-I T cells were cocultured with control mAbs- or anti-DR5 mAbs-cross-linked BM-DCs and γ-ray irradiated P815/tOVA in the presence of inhibitors, including 50 μM L-NIL, 50 μM nor-NOHA, 200 μM 1-MT, media control, or in the presence of blocking mAbs against IL-10 (10 μg/ml) or control mAbs. After 4 days, proliferation of CD8β⁺ T cells was determined by flow cytometry (Media or IgG2a = 100%). Cumulative data from three independent experiments are shown as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.