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. 2000 Oct 1;14(19):2486–2500. doi: 10.1101/gad.831500

Figure 1.

Figure 1

Phenotypes of unc-130 mutants. (A) The distal tip cell (DTC) migration (Mig) phenotype. In unc-130(ev505), L4 hermaphrodites grown at 25°C, the DTCs often fail to undergo the second, ventral-to-dorsal, phase of their migration leading to ventral clear patches (black arrows) flanking the vulva, caused by the ventral misplacement of the distal arm of the gonad. The DTCs act independently and show incomplete penetrance for the Mig phenotype, producing hermaphrodites with posterior (a), anterior (b), posterior and anterior (c), or no (not shown) ventral clear patches. (B) DA and DB motor neurons in wild-type adult animals grown at 25°C. DA and DB motor neurons visualized using evIs82 (neuronal-specific unc-129::gfp). These motor neurons extend growth cones from their cell bodies in the ventral nerve cord (white arrows) into the dorsal nerve cord (White et al. 1986). (C) unc-130(ev505); evIs82 adult hermaphrodite grown at 25°C. The DA and DB motor neurons often have axons with abnormal morphologies (white arrows). Instead of projecting directly from the ventral to dorsal nerve cord, axons often fail to reach the dorsal cord and run longitudinally at abnormal lateral positions. These aberrant axons indicate a defect in ventral-to-dorsal growth cone guidance. (D) DD and VD motor neurons visualized by staining an evIs113 (unc-5::lacZ; rol-6) adult hermaphrodite grown at 25°C for β-galactosidase activity (see Materials and Methods). As with the DA and DB motor neurons, the VD and DD motor neurons extend pioneer growth cones from the ventral nerve cord (black arrowhead) to the dorsal nerve cord (out of focus). (E) unc-130(ev505); evIs113 (unc-5::lacZ; rol-6) adult hermaphrodite grown at 25°C. DD and VD motor axons (white arrowheads) often fail to reach the dorsal nerve cord in unc-130 mutants. (F) Embryonic phenotype in unc-130(ev505). Embryos show variable defects. Cells often are found outside the anterior of the main embryo mass (black arrow) just before body elongation, which may be indicative of a defect in closure of the head epidermis. (G) unc-130(ev505) hatchling. L1 hatchlings sometimes have dorsal head bumps (black arrow). (H) him-5(e1490) male tail. Wild-type and control him-5(e1490) male tails have nine sensory rays on each side of the fan. Each ray (numbered 1–9) has a distinct position and morphology. (I) unc-130(ev505); him-5(e1490) male tail. unc-130 mutant males have variable fusions of sensory rays in the male tail. Shown here is a male tail with fusion of ray 3 with rays 4 and 6, and ray 7 with rays 8 and 9.