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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2011 Aug 12;187(6):2853–2858. doi: 10.4049/jimmunol.1101143

FIGURE 1. Generation of Igα serine and threonine mutant mice.

FIGURE 1

A, Amino acid sequence of the murine Igα cytoplasmic domain.

B, Scheme of mutant Igα protein expressed as part of the BCR complex in IgαSATV/SATV mice.

C, Splenic B cells were stimulated with 5μg/106 cells anti-IgM for the indicated time points, lysed with 1%NP-40 buffer, separated by SDS-PAGE, and blotted as indicated. Bands were quantified after digital chemiluminescence acquisition. Blots shown are representative of four experiments performed with lysates from two independent experiments with three to four 8-12 week old mice each.