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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2011 Aug 12;187(6):2853–2858. doi: 10.4049/jimmunol.1101143

FIGURE 4. Plasma cell formation and Ig isotype switching.

FIGURE 4

A, Antibody forming cells in the bone marrow of one femur were quantified in 7-10 week old mice (n=9-13) in 5 independent experiments by ELISPOT analysis. Graphs show values from individual mice, medians and p-values as calculated by Student's T-test.

B, Ig class switching to IgG2c and IgG2b was determined by culture of splenic B cells from three 8 week old mice with 20μg/ml LPS for 4 days, staining with anti-IgG2c and IgG2b Ig and loss of CFSE by flow cytometry. Numbers shown represent the percentage of gated cells. IgG2c and IgG2b secretion by LPS stimulated B cells into the supernatants was determined by ELISA, and depicted as ratio of Ig to number of B cells plated on day 0. Results were comparable in a second independent experiment.

C, The fraction of IgG2c and IgG2b expressing GC cells in mesenteric lymph nodes and PPs were determined in 8-12 week old mice by flow cytometry (n=5-6) in two independent experiments. Histograms display averages and standard deviations.

D, Antibody forming cells in the spleen were quantified in 7-10 week old mice (n=8-18) by ELISPOT analysis. Graphs show values from individual mice, medians and p-values as calculated by Student's T-test.