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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2011 Aug 19;187(6):3256–3266. doi: 10.4049/jimmunol.1002915

Fig. 6. Regulation of NF-κB by c-FLIP is upstream of IKK.

Fig. 6

A. Cells transfected with either pcDNA control plasmid or 4ng/ml c-FLIP and exposed to 200ng/ml FasL in the absence or presence of 10mM NAC were probed for phosphorylated IKKα/β using western blotting. Blots were re-probed with GAPDH antibody to confirm equal loading of the samples. B, C. Cells were co-transfected with 100ng/well of NF-κB-Luc plasmid, 10ng/well of pRL-tk plasmid, 4ng/ml of either pcDNA3 or c-FLIP plasmids and 1 μg pcDNA3, IKK-WT or IKK-DN plasmids. 24 h post transfection, cells were treated with 200ng/ml FasL along with (B) 10mM NAC or (C) 20μM LY294002. Treated cells were analyzed for NF-κB levels by luciferase assay. Plots show relative NF-κB levels over non-treated control. Values are mean ± SD (n = 4). *P < 0.05 versus non-treated control. **P < 0.05 versus pcDNA-transfected control datasets.