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. 1998 Jul 1;12(13):2061–2072. doi: 10.1101/gad.12.13.2061

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

 Analysis of the interactions between HPV E6 proteins and interferon regulatory factors (A) The indicated HPV E6 proteins were in vitro-translated in the presence of [35S]cysteine and methionine and mixed with GST–IRF-3 immobilized on glutathione–Sepharose. The complexes were washed to removed noninteracting proteins and resolved by PAGE. Input corresponds to 10% of protein used in the binding experiments. (B) IRF-3 was synthesized in rabbit reticulocyte lysate in the presence of [35S]cysteine and methionine and mixed with indicated GST alone or GST–E6 immobilized on glutathione–Sepharose. Complexes were washed and resolved by PAGE. Input corresponds to 50% of the protein used in the binding reactions. (C) COS-7 cells were electroporated with indicated constructs and after 36 hr, lysates were immunoprecipitated with control C, AU1, or IRF-3 (SL-12) monoclonal antibodies. HPV16 E6 and the light chain are indicated by arrows. (D) Indicated IRF proteins were synthesized in rabbit reticulocyte lysate in the presence of [35S]cysteine and methionine and mixed with either GST or GST–E6 immobilized on glutatione–Sepharose. Complexes were washed and resolved by PAGE. Input corresponds to 50% of protein.