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. 2011 Apr 7;18(9):929–935. doi: 10.1038/gt.2011.41

Figure 3.

Figure 3

Hydrodynamic delivery of shRNA and U1i constructs to murine liver inhibits luciferase activity in vivo. Mice were hydrodynamically co-transfected with 10 μg shRNA (shLuc1 or control shApoB) and 40 μg double U1i (L4/L5 or control H1/H4) plasmid and with 2 μg firefly luciferase reporter and 0.5 μg secreted alkaline phophatase (SEAP) plasmid. Both the firefly luciferase and SEAP reporter gene were expressed under control of the liver-specific AAT promoter, as hydrodynamically delivered plasmid DNA localizes mainly to the liver. Two days after transfection, bioluminescence was measured in the IVIS, and luciferase signal was calculated relative to plasma SEAP levels. Murine liver co-transfected with control constructs shApoB and H1/H4 was set at 100%. Co-transfection of shLuc1 with L4/L5 maximally reduced luciferase activity by 84%. However, luciferase knockdown was not significantly different between treatments. Data are presented as mean (n=4–8)±s.e.