Figure 2.

Global immunofluorescence analysis of Spt5 and Spt6 on polytene chromosomes under non–heat shock conditions. (A) Costaining with different Spt5 antibodies. (Rab) Rabbit; (gp) guinea pig. (B) Costaining with different Spt6 antibodies. (C) Costaining with Spt5 (rab) and Spt6 (gp) antibodies. (D) Costaining with Spt6 (gp) and cyclin T (rab) antibodies. (E) Costaining with Spt6 (gp) and Pol II (rab) antibodies. Overlap of the green and red stains appear in the merge as yellow, yellow-green, and orange.