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. 2000 Oct 15;14(20):2635–2649. doi: 10.1101/gad.844200

Figure 3.

Figure 3

Global and high-resolution analysis of Spt6 at native and transgenic heat shock puffs. (A) Comparative immunofluorescence of Spt6 (rat) and HSF (rabbit) under heat shock conditions. Spt6 is recruited to heat shock loci but not to all sites of HSF localization. (B,C) High-resolution study of Spt6 distribution in comparison to that of HSF, cyclin T, and Pol II. Maps of the native heat shock loci 87A and 87C and the hsp70–LacZ transgene are shown. HSF and cyclin T resolve from Spt6 at both the native and transgenic heat shock loci. At the transgene, these antibodies stain the locus in a region that would be consistent with promoter and 5′ sequences. Note the HSF staining pattern (white arrow). The site above 87A and 87C in the cyclin T and Spt6 costain is the heat shock locus 67B. Spt6 and Pol II show almost complete overlap within the heat shock puffs. (D) Triple label with cyclin T, HSF, and Spt6.