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. 2000 Oct 15;14(20):2635–2649. doi: 10.1101/gad.844200

Figure 4.

Figure 4

Crosslinking and immunoprecipitation of Spt5 and Spt6 at hsp70. (A) Schematic representation of hsp70 and the PCR-amplified fragments representing the upstream region (1), the promoter (2), the 5′ end (3), and the 3′ end (4) of hsp70. The promoter elements, transcription start site, and stop are labeled but not drawn to scale. (B) PCR analysis of crosslinked and immunoprecipitated DNA on ethidium bromide (EtBr)-stained 2% agarose gels. PCR reactions using 10%, 1%, and 0.1% of input DNAs are loaded to determine the linear range of signal. NHS, non–heat shock; (HS) heat shock; (Pre) preimmune serum; (−) no DNA. (C) Quantitative analysis of EtBr-stained bands for PCR analysis. Values on the abscissa represent the amount immunoprecipitated as a percentage of total input DNA. Experiments were performed in triplicate, and standard deviations are shown. Note different scale for bar graph of fragment 3 immunoprecipitates.