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. Author manuscript; available in PMC: 2011 Sep 9.
Published in final edited form as: J Am Chem Soc. 2007 Sep 18;129(40):12123–12130. doi: 10.1021/ja072130e

Figure 7.

Figure 7

In vitro replication studies of N2-CEdG-bearing and control undamaged substrates with Klenow fragment of E. coli polymerase I (X represents S-N2-CEdG, R-N2-CEdG or unmodified dG). The concentrations of the primer and template were 5 and 10 nM, respectively, and the reaction volume was 20 μL. (a) The primer extension was carried out in the presence of all four dNTPs at a concentration of 200 μM each, and the amounts Kf− were as indicated in the figure. The reactions were continued at 37°C for 60 min. (b) The reaction was performed in the presence of one type of dNTP at a time ([dNTP] =1.0 mM) at 37°C for 10 min, and 0.1 unit of Kf− was used.