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. Author manuscript; available in PMC: 2011 Sep 9.
Published in final edited form as: J Am Chem Soc. 2007 Sep 18;129(40):12123–12130. doi: 10.1021/ja072130e

Table 2.

Thermodynamic Parameters for Duplex Formation

duplex Tma (°C) ΔH (kcal mol−1) ΔS (cal mol−1 K−1) ΔG25 °C (kcal mol−1) ΔΔG25 °Cb (kcal mol−1)
5′-CTCATCCTACTC-3′
3′-GAGTAXGATGAG-5′
X = G 43.6 ± 0.1 −87.0 ± 4.5 246 ± 16 −13.7 ± 0.6
X = S-N2-CEG 29.9 ± 0.2 −77.7 ± 1.0 228 ± 4 −9.9 ± 0.2 3.8
X = R-N2-CEG 29.2 ± 0.1 −72.2 ± 1.9 210 ± 6 −9.7 ± 0.3 4.0
a

Ct = 2.0 μM.

b

ΔΔG25 °C = ΔG25 °C (lesion-containing duplex) - ΔG25 °C (unmodified duplex)