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. 2000 Oct 15;14(20):2650–2663. doi: 10.1101/gad.836400

Figure 4.

Figure 4

hB′′ is required for RNA polymerase III transcription from the Ad2 VAI and human U6 promoters. (A) Whole-cell extract was treated with beads coated with either preimmune (lane 1) or anti-hB′′ (lanes 2–5) antibodies and tested for transcription from the Ad2 VAI promoter. In lanes 3–5, increasing amounts of rhB′′ (rhB′′) expressed in E. coli were added to the depleted extract. Lane 6 shows VAI transcription in untreated whole-cell extract. (B) Whole-cell extract treated with beads coated with the preimmune (lane 1) or anti-hB′′ (lanes 2–4) antibodies and tested for U6 transcription. In lanes 3 and 4, increasing amounts of rhB′′ expressed in E. coli were added to the depleted extract. Lane 5 shows U6 transcription in untreated whole-cell extract. (IC) internal control signal.