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hBRFU is expressed in HeLa cells. The immunoblot was performed with an anti-hBRFU/hBRF antibody (CS1043). (Lane 1) rhBRFU expressed in E. coli; (lane 2) rhBRFU expressed in E. coli and 2 μL of P11 B fraction; and (lane 3) 2 μL of P11 B fraction. rhBRFU migrates as a protein of ∼50 kD. The additional bands seen in the P11 fraction probably correspond to cross-reacting proteins.
