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The λ N protein binds directly to the 192 carboxy-terminal amino acids of NusA. E. coli extract containing additional NusA (lane 1), NusA (amino acids 1–399) (lane 4), or NusA (amino acids 303–495) (lane 7) was passed over columns containing 2 mg/ml GST (lanes 2, 5, and 8) or 0.5 mg/ml GST–N (lanes 3, 6, and 9). Bound proteins were eluted with buffer containing 1M NaCl, subjected to SDS-PAGE, and stained with silver.
